LOCUS       pDG2i-tdTomato-V5-puro       11295 bp    DNA     circular UNA 20-NOV-2015
DEFINITION  LMBP 7945.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7945
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 20-NOV-2015
                       
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|751044614|
COMMENT     VNTDBDATE|751044614|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pDG2i-tdTomato-V5 puro|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pDG2i-tdTomato-V5 puro" 0 11295 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
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COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 8677 9252 1 11295 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT            (CStringList) 1 1 1 1 "<7929..8626") 
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COMMENT            0 10118 10690 0 (CStringList) (CStringList) 1 1 1 1 "<8692..9267") 
COMMENT        #2=(CFSignal (CObList) "puromycin resistance" 4 0 0 10693 11295 0 
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COMMENT        #3=(CFSignal (CObList) "SV40 polyA early" 43 0 0 397 471 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4469..4543") 
COMMENT        #4=(CFSignal (CObList) "RSV/HIV-1 5' LTR" 29 0 0 3653 3981 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "7725..8053") 
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COMMENT            (CStringList) 1 1 1 1 "4972..5427") 
COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 0 1486 2346 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "5558..6418") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 0 2887 3289 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "6959..7361") 
COMMENT        #8=(CFSignal (CObList) "lac promoter" 30 0 0 3381 3464 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "7453..7536") 
COMMENT        #9=(CFSignal (CObList) "T3 promoter" 30 0 0 3528 3544 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "7600..7616") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 397 471 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "RSV/HIV-1 dU3/3' LTR" 43 0 0 91 325 0 
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COMMENT        #18=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 5940 6599 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "268..927") 
COMMENT        #19=(CFSignal (CObList) "ccdB" 4 0 0 6941 7246 0 (CStringList) 
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COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #24=(CFSignal (CObList) "loxP" 21 0 0 10062 10095 0 (CStringList) 
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COMMENT        #25=(CFSignal (CObList) "V5 epitope" 22 0 0 8889 8930 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #26=(CFSignal (CObList) "tdTomato" 4 0 0 7443 8870 0 (CStringList) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 11/20/15 03:30" 1) 
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COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                                   "HIV-1 Rev response element" "@N" 1 13.8169 
COMMENT                                   -13.9386 0 -10 12.416 1.12386 #246#) 
COMMENT                             #248=(CScratch 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #166# 1005 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 1 6.23209 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #249=(CLabel (CWidget 0 (0 0) 1 2 0 0 #204# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "HIV-1 cPPT" 
COMMENT                                   "@N" 1 -2.2426 16.8881 0 -10 5.28035 1.12386 
COMMENT                                   #248#) 
COMMENT                             #250=(CScratch 
COMMENT                                   (CWidget 0 (3 #24# 0) 1 2 0 0 #166# 786 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 1 0.685357 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #251=(CLabel (CWidget 0 (0 0) 1 2 0 0 #204# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 
COMMENT                                   -11.0583 12.1963 0 -10 1.89094 1.12386 #250#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #252=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -257 100) 
COMMENT                            (CObjectList 
COMMENT                             #253=(CScratch 
COMMENT                                   (CWidget 0 (3 #25# 0) 1 2 0 0 #202# 0 100) 
COMMENT                                   (LOGPEN 0 6 10040064) 6 1 1.32573 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #254=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "V5 epitope" 
COMMENT                                   "@N" 1 -17.6825 4.25174 0 -10 4.74518 1.12386 
COMMENT                                   #253#)) (CObjectList)) 
COMMENT                      #255=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -538 100) 
COMMENT                            (CObjectList 
COMMENT                             #256=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #200# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.997848 4.06216 4.24488 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #257=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #203# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "RSV/HIV-1 5' LTR" "@N" 1 17.0048 -8.71165 0 
COMMENT                                   -10 7.90268 1.12386 #256#) 
COMMENT                             #258=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #200# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    3.12743 3.27627 1 0.082322) 0.8 1.8 0) 
COMMENT                             #259=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hCMVTRE promoter" "@N" 1 5.28249 -16.4659 0 
COMMENT                                   -10 8.79463 1.12386 #258#) 
COMMENT                             #260=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #22# 0) 1 2 0 0 #200# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.835356 1.13856 1.29574 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #261=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early promoter" "@N" 1 -19.1507 5.58377 
COMMENT                                   0 -10 9.08006 1.12386 #260#)) (CObjectList)) 
COMMENT                      #262=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -889 100) 
COMMENT                            (CObjectList 
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COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #182# 0 100) 
COMMENT                                    (LOGPEN 0 6 128) 6 0.723062 4.30868 4.39595) 
COMMENT                                   0.4 0.082322 0.817773 1.8 1.8 0) 
COMMENT                             #264=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 16.998 -5.22699 0 -10 5.51225 1.12386 
COMMENT                                   #263#) 
COMMENT                             #265=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #182# 0 100) 
COMMENT                                    (LOGPEN 0 6 128) 6 0.820673 4.22704 4.31431) 
COMMENT                                   0.4 0.082322 0.948704 1.8 1.8 0) 
COMMENT                             #266=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T3 promoter" 
COMMENT                                   "@N" 1 16.4892 -6.96932 0 -10 5.33386 1.12386 
COMMENT                                   #265#) 
COMMENT                             #267=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #182# 0 100) 
COMMENT                                    (LOGPEN 0 6 128) 6 0.835356 5.87608 5.96334) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #268=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 7.66555 11.4589 0 -10 5.33386 1.12386 
COMMENT                                   #267#)) (CObjectList)) 
COMMENT                      #269=(CGroupWidget 
COMMENT                            (CWidget 32 (7 32 0) 1 2 0 0 Nil -327 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWidget 0 (3 #1# 0) 1 2 0 0 #180# 1332 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 0.835356 0.336014 
COMMENT                                   0.654255) 
COMMENT                             #271=(CLabel (CWidget 0 (0 0) 1 2 0 0 #204# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of the EMCV polyprotein gene (IRES)" 
COMMENT                                   "@N" 1 -16.5741 13.4748 0 -10 19.5516 1.12386 
COMMENT                                   #270#)) (CObjectList)) 
COMMENT                      #272=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -397 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #5# 0) 1 2 0 0 #175# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    5.52063 5.77389 1 0.082322) 0.8 1.8 0) 
COMMENT                             #274=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 origin" "@N" 
COMMENT                                   1 9.00262 10.0733 0 -10 3.67484 1.12386 #273#) 
COMMENT                             #275=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #175# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    4.44649 4.67032 1 0.082322) 0.8 1.8 0) 
COMMENT                             #276=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 17.0592 -0.957414 0 -10 4.03162 1.12386 
COMMENT                                   #275#) 
COMMENT                             #277=(CScratch 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #168# 786 100) 
COMMENT                                   (LOGPEN 0 10 8388608) 10 1 5.93662 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #278=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 6.48734 12.8266 0 -10 3.90674 1.12386 #277#) 
COMMENT                             #279=(CScratch 
COMMENT                                   (CWidget 0 (3 #23# 0) 1 2 0 0 #168# 0 100) 
COMMENT                                   (LOGPEN 0 10 8388608) 10 1 1.163 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #280=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 -16.485 7.09419 0 -10 3.90674 1.12386 #279#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #281=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -257 100) 
COMMENT                            (CObjectList 
COMMENT                             #282=(CScratch 
COMMENT                                   (CWidget 0 (3 #3# 0) 1 2 0 0 #166# 1005 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 1 6.05325 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #283=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #213# -858993459 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 7.21017 15.4371 0 
COMMENT                                   -10 7.65294 1.12386 #282#) 
COMMENT                             #284=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #166# 1026 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 1 6.05325 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 7.19955 14.2121 0 
COMMENT                                   -10 7.01073 1.12386 #284#) 
COMMENT                             #286=(CLine 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #166# 1332 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 0.835356 6.09268 
COMMENT                                   6.2232) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 65 24 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "RSV/HIV-1 dU3/3' LTR" "@N" 1 7.05822 16.8583 
COMMENT                                   0 -10 9.91849 1.12386 #286#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #288=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -468 100) 
COMMENT                     (CObjectList 
COMMENT                      #289=(CLabel (CWidget 0 (15 #27# 0) 1 2 0 0 #188# 0 100) 
COMMENT                            (LOGPEN 0 0 32896) 1 
COMMENT                            (LOGFONT 59 22 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 20-NOV-2015" 
COMMENT                            "Last modified: 20-NOV-2015" 65535 -0.071356 
COMMENT                            -4.56679 0 -10 12.0235 1.07034 Nil) 
COMMENT                      #290=(CLabel (CWidget 0 (15 #28# 0) 1 2 0 0 #188# 2 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 88 33 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 7945" 
COMMENT                            "LMBP 7945" 65535 0 -2.6045 0 -10 7.2248 1.55199 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #291=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -1100 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #292=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1030 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) -2147469808 
COMMENT       (CStringList) 2147483648 268632118 (CObList)))
FEATURES             Location/Qualifiers
     CDS             9354..10052
                     /vntifkey="4"
                     /label=rtTA3
     RBS             10118..10690
                     /vntifkey="32"
                     /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES)
     CDS             10693..11295
                     /vntifkey="4"
                     /label=puromycin\resistance
     terminator      397..471
                     /vntifkey="43"
                     /label=SV40\polyA\early
     promoter        3653..3981
                     /vntifkey="29"
                     /label=RSV/HIV-1\5'\LTR
     rep_origin      900..1355
                     /vntifkey="33"
                     /label=f1\origin
     CDS             1486..2346
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      2887..3289
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3381..3464
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        3528..3544
                     /vntifkey="30"
                     /label=T3\promoter
     terminator      complement(397..471)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      607..682
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(716..732)
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    4092..4136
                     /vntifkey="21"
                     /label=HIV-1\psi\packaging\signal
     misc_feature    4646..4879
                     /vntifkey="21"
                     /label=HIV-1\Rev\response\element
     terminator      91..325
                     /vntifkey="43"
                     /label=RSV/HIV-1\dU3/3'\LTR
     misc_feature    75..90
                     /vntifkey="21"
                     /label=HIV-1\cPPT
     promoter        5397..5664
                     /vntifkey="29"
                     /label=hCMVTRE\promoter
     CDS             5940..6599
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             6941..7246
                     /vntifkey="4"
                     /label=ccdB
     attenuator      7287..7411
                     /vntifkey="0"
                     /label=attR2
     attenuator      complement(5707..5831)
                     /vntifkey="0"
                     /label=attR1
     promoter        8963..9245
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      9202..9277
                     /vntifkey="33"
                     /label=SV40\ori
     misc_feature    10062..10095
                     /vntifkey="21"
                     /label=loxP
     misc_marker     8889..8930
                     /vntifkey="22"
                     /label=V5\epitope
     CDS             7443..8870
                     /vntifkey="4"
                     /label=tdTomato
BASE COUNT     2879 a      2868 c      2946 g      2602 t 
ORIGIN
        1 gctaagcaca attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat 
       61 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga 
      121 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 
      181 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 
      241 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 
      301 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 
      361 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 
      421 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 
      481 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 
      541 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
      601 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 
      661 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag 
      721 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 
      781 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 
      841 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga 
      901 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
      961 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 
     1021 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 
     1081 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 
     1141 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
     1201 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
     1261 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
     1321 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg 
     1381 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 
     1441 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 
     1501 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 
     1561 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 
     1621 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 
     1681 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 
     1741 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 
     1801 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 
     1861 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 
     1921 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 
     1981 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 
     2041 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 
     2101 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 
     2161 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 
     2221 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 
     2281 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 
     2341 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 
     2401 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 
     2461 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     2521 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     2581 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     2641 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 
     2701 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     2761 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     2821 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 
     2881 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 
     2941 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 
     3001 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 
     3061 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 
     3121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 
     3181 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 
     3241 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 
     3301 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 
     3361 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 
     3421 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 
     3481 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac 
     3541 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt 
     3601 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg 
     3661 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg 
     3721 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt 
     3781 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct 
     3841 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 
     3901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 
     3961 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc 
     4021 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg 
     4081 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg 
     4141 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt 
     4201 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct 
     4261 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact 
     4321 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac 
     4381 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt 
     4441 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga 
     4501 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata 
     4561 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc 
     4621 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag 
     4681 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg 
     4741 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc 
     4801 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc 
     4861 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg 
     4921 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga 
     4981 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg 
     5041 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg 
     5101 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa 
     5161 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata 
     5221 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac 
     5281 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc 
     5341 gattagtgaa cggatctcga cggtatcgat ggtccgaggt tctagacgag tttactccct 
     5401 atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga 
     5461 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag 
     5521 aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat 
     5581 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag 
     5641 ctcgtttagt gaaccgtcag atcgcaccgg tcactagtcc agtgtggtgg aattctgcag 
     5701 atatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat 
     5761 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc 
     5821 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt 
     5881 ataatgtgtg gattttgagt taggatccgg cgagattttc aggagctaag gaagctaaaa 
     5941 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac 
     6001 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata 
     6061 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc 
     6121 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg 
     6181 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa 
     6241 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt 
     6301 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga 
     6361 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg 
     6421 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg 
     6481 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg 
     6541 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa 
     6601 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt 
     6661 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat 
     6721 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat 
     6781 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt 
     6841 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa 
     6901 atgaacggct cttttgctga cgagaacagg gactggtgaa atgcagttta aggtttacac 
     6961 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac 
     7021 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc 
     7081 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga 
     7141 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga 
     7201 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccgt 
     7261 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt 
     7321 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg 
     7381 tttctcgttc agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga 
     7441 gtatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg 
     7501 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 
     7561 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg 
     7621 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca 
     7681 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 
     7741 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga 
     7801 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga 
     7861 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg 
     7921 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga 
     7981 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc 
     8041 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg 
     8101 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca 
     8161 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc 
     8221 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 
     8281 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg 
     8341 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg 
     8401 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 
     8461 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca 
     8521 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc 
     8581 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc 
     8641 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt 
     8701 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca 
     8761 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct 
     8821 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag gagggcccgc 
     8881 ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt 
     8941 agtaatgagt ttggaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 
     9001 aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 
     9061 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 
     9121 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 
     9181 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 
     9241 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 
     9301 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg accatgtcta 
     9361 ggctggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg 
     9421 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact 
     9481 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc 
     9541 atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg 
     9601 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca 
     9661 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc 
     9721 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg 
     9781 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca 
     9841 ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg 
     9901 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt 
     9961 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg 
    10021 atgcccttga cgattttgac cttgacatgc tccccgggta aataacttcg tatagtatac 
    10081 attatacgaa gttatggatc cgcggccgca aattccgccc ctctccctcc ccccccccta 
    10141 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt 
    10201 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga 
    10261 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg 
    10321 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt 
    10381 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat 
    10441 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg 
    10501 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg 
    10561 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt 
    10621 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 
    10681 cacgataata ccatggccac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 
    10741 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 
    10801 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 
    10861 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 
    10921 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg 
    10981 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 
    11041 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 
    11101 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg 
    11161 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 
    11221 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 
    11281 aagcccggtg cctga 
//
