LOCUS       pDG1i-eGFP-V5-blast        9098 bp    DNA     circular     20-NOV-2015
DEFINITION  LMBP 7941.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7941
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 20-NOV-2015
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|743855048|
COMMENT     VNTDBDATE|751029441|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pDG1i-eGFP-V5 blast|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pDG1i-eGFP-V5 blast" 0 9098 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 9098 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "V5 epitope" 22 0 0 2506 2547 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2506..2547") 
COMMENT        #1=(CFSignal (CObList) "synthetic EM7 promoter" 30 0 0 2967 3032 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2965..3032") 
COMMENT        #2=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 268 927 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "268..927") 
COMMENT        #3=(CFSignal (CObList) "ccdB" 4 0 0 1269 1574 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1269..1574") 
COMMENT        #4=(CFSignal (CObList) "blasticidin resistance" 4 0 0 3033 3431 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3033..3431") 
COMMENT        #5=(CFSignal (CObList) "attR2" 0 0 0 1615 1739 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1615..1739)") 
COMMENT        #6=(CFSignal (CObList) "attR1" 0 0 1 35 159 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "35..159") 
COMMENT        #7=(CFSignal (CObList) "EGFP" 4 0 0 1771 2487 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1771..2487") 
COMMENT        #8=(CFSignal (CObList) "SV40 early promoter" 29 0 0 2580 2862 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "SV40 ori" 33 0 0 2819 2894 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3823 3897 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4469..4543") 
COMMENT        #11=(CFSignal (CObList) "RSV/HIV-1 5' LTR" 29 0 0 7079 7407 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "7725..8053") 
COMMENT        #12=(CFSignal (CObList) "f1 origin" 33 0 0 4326 4781 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4972..5427") 
COMMENT        #13=(CFSignal (CObList) "ampicillin resistance" 4 0 0 4912 5772 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5558..6418") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 0 6313 6715 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6959..7361") 
COMMENT        #15=(CFSignal (CObList) "lac promoter" 30 0 0 6807 6890 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7453..7536") 
COMMENT        #16=(CFSignal (CObList) "T3 promoter" 30 0 0 6954 6970 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7600..7616") 
COMMENT        #17=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3823 3897 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4469..4543)") 
COMMENT        #18=(CFSignal (CObList) "SV40 ori" 33 0 0 4033 4108 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4679..4754") 
COMMENT        #19=(CFSignal (CObList) "T7 promoter" 30 0 1 4142 4158 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4788..4804)") 
COMMENT        #20=(CFSignal (CObList) "HIV-1 psi packaging signal" 21 0 0 7518 7562 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "8164..8208") 
COMMENT        #21=(CFSignal (CObList) "HIV-1 Rev response element" 21 0 0 8072 8305 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "8718..8951") 
COMMENT        #22=(CFSignal (CObList) "RSV/HIV-1 dU3/3' LTR" 43 0 0 3517 3751 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4163..4397") 
COMMENT        #23=(CFSignal (CObList) "HIV-1 cPPT" 21 0 0 3501 3516 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4147..4162") 
COMMENT        #24=(CFSignal (CObList) "hCMVTRE promoter" 29 0 0 8823 9090 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #25=(CFSignal (CObList) "LMBP 7941" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #26=(CFSignal (CObList) "Last modified: 20-NOV-2015" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #27=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #28=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "pDG1i-eGFP-V5 blast" 2) 5 "" 0 4) 
COMMENT              #29=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pDG1i-eGFP-V5 blast'" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 7941." 
COMMENT                          1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 09/01/15 10:24" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 11/20/15 11:17" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9098 bp" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 1 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 1-SEP-2015" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #41=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #42=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #43=(CLinePar (CParagraph 0 (15 #25# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 7941") 
COMMENT                     #44=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #45=(CLinePar (CParagraph 0 (15 #26# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 20-NOV-2015"))) 
COMMENT                   "Annotations") 
COMMENT              #46=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #47=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #48=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (7 0 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 35 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 35    End: 159  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   35..159" 1))) "attR1") 
COMMENT                            #53=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1615 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1615  End: 1739" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1615..1739)" 1))) "attR2"))) 
COMMENT                          "Attenuator (2 total)") 
COMMENT                     #57=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 268 (3 #2# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 268   End: 927 " 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   268..927" 1))) 
COMMENT                                 "chloramphenicol resistance") 
COMMENT                            #62=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1269 (3 #3# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1269  End: 1574" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1269..1574" 1))) "ccdB") 
COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1771 (3 #7# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1771  End: 2487" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1771..2487" 1))) "EGFP") 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3033 (3 #4# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3033  End: 3431" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3033..3431" 1))) 
COMMENT                                 "blasticidin resistance") 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4912 (3 #13# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4912  End: 5772" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5558..6418" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (5 total)") 
COMMENT                     #78=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3501 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3501  End: 3516" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4147..4162" 1))) "HIV-1 cPPT") 
COMMENT                            #83=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7518 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7518  End: 7562" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   8164..8208" 1))) 
COMMENT                                 "HIV-1 psi packaging signal") 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 8072 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 8072  End: 8305" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   8718..8951" 1))) 
COMMENT                                 "HIV-1 Rev response element"))) 
COMMENT                          "Misc. Feature (3 total)") 
COMMENT                     #91=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #92=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2506 (3 #0# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2506  End: 2547" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2506..2547" 1))) "V5 epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #96=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #97=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2580 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2580  End: 2862" 1))) 
COMMENT                                 "SV40 early promoter") 
COMMENT                            #99=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7079 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Start: 7079  End: 7407" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   7725..8053" 1))) "RSV/HIV-1 5' LTR") 
COMMENT                            #103=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 8823 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 8823  End: 9090" 1))) 
COMMENT                                  "hCMVTRE promoter"))) 
COMMENT                          "Promoter Eukaryotic (3 total)") 
COMMENT                     #105=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #106=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2967 (3 #1# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #107=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2967  End: 3032" 1) 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2965..3032" 1))) 
COMMENT                                   "synthetic EM7 promoter") 
COMMENT                             #110=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4142 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4142  End: 4158 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(4788..4804)" 1))) 
COMMENT                                   "T7 promoter") 
COMMENT                             #114=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6807 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6807  End: 6890" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   7453..7536" 1))) "lac promoter") 
COMMENT                             #118=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6954 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6954  End: 6970" 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   7600..7616" 1))) "T3 promoter"))) 
COMMENT                           "Promoter Prokaryotic (4 total)") 
COMMENT                     #122=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #123=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2819 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2819  End: 2894" 1))) 
COMMENT                                   "SV40 ori") 
COMMENT                             #125=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4033 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4033  End: 4108" 1) 
COMMENT                                     #127=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4679..4754" 1))) "SV40 ori") 
COMMENT                             #129=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4326 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4326  End: 4781" 1) 
COMMENT                                     #131=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #132=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4972..5427" 1))) "f1 origin") 
COMMENT                             #133=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6313 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #134=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6313  End: 6715" 1) 
COMMENT                                     #135=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #136=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   6959..7361" 1))) "pMB1 ori"))) 
COMMENT                           "Replication Origin (4 total)") 
COMMENT                     #137=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #138=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3517 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #139=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3517  End: 3751" 1) 
COMMENT                                     #140=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #141=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4163..4397" 1))) 
COMMENT                                   "RSV/HIV-1 dU3/3' LTR") 
COMMENT                             #142=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3823 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #143=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3823  End: 3897" 1) 
COMMENT                                     #144=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #145=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4469..4543" 1))) 
COMMENT                                   "SV40 polyA early") 
COMMENT                             #146=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3823 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #147=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3823  End: 3897 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #148=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #149=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(4469..4543)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #150=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #151=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #152=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #153=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #154=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #155=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #156=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #157=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #158=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #159=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #160=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #161=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #162=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #163=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #164=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #165=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #166=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #167=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) #168=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #169=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #170=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #171=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #172=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #173=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #174=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #175=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #176=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #177=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #178=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #179=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #180=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #181=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #182=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #183=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #184=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #185=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #186=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #187=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #188=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #189=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #190=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #191=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #192=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #193=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #194=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #195=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #196=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #197=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #198=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #199=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #200=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #201=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #202=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #203=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #204=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #205=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #206=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #207=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #208=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #209=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -3261) 1 1 0 1 1 
COMMENT        (mapper: 24.976 -15.2375 63.3864 63.3864 0.01 10 -1 9098 9098 1 0 0) 
COMMENT        #210=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 745434985 100) 
COMMENT              (CObjectList 
COMMENT               #211=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -686 100) 
COMMENT                     (CObjectList 
COMMENT                      #212=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #167# 98592932 0) 
COMMENT                             (LOGPEN 0 2 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27487 
COMMENT                             6.27287 1 0.0214037) 0.0689513) 
COMMENT                      #213=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #160# 0 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 100 38 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pDG1i-eGFP-V5 blast" "@N" 1 0 
COMMENT                            0.871165 0 -10 12.9839 1.46719 Nil) 
COMMENT                      #214=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #159# 982554446 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 115 43 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "9098 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 4.93797 1.73539 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #215=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -686 100) 
COMMENT                     (CObjectList 
COMMENT                      #216=(CGroupWidget 
COMMENT                            (CWidget 0 (7 0 0) 1 2 0 0 Nil -1311 100) 
COMMENT                            (CObjectList 
COMMENT                             #217=(CWideLine 
COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #190# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 (LOGBRUSH 0 16750848 0) 1 
COMMENT                                   5.07412 5.16031 1 0.082322) 
COMMENT                             #218=(CLabel (CWidget 0 (0 0) 1 2 0 0 #186# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "attR2" "@N" 1 
COMMENT                                   13.6657 5.68676 0 -10 2.4611 1.15167 #217#) 
COMMENT                             #219=(CWideLine 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #190# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 (LOGBRUSH 0 16750848 0) 1 
COMMENT                                   6.16355 6.24974 1 0.082322) 
COMMENT                             #220=(CLabel (CWidget 0 (0 0) 1 2 0 0 #186# 1 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "attR1" "@N" 1 
COMMENT                                   2.26171 12.6517 0 -10 2.4611 1.15167 #219#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #221=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -1381 100) 
COMMENT                            (CObjectList 
COMMENT                             #222=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #163# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    5.63401 6.08909 1 0.082322) 0.8 1.8 0) 
COMMENT                             #223=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 2082 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "chloramphenicol resistance" "@N" 1 11.1134 
COMMENT                                   12.0476 0 -10 12.195 1.15167 #222#) 
COMMENT                             #224=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #163# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    5.18789 5.39888 1 0.082322) 0.8 1.8 0) 
COMMENT                             #225=(CLabel (CWidget 0 (0 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ccdB" "@N" 1 
COMMENT                                   12.8721 7.88885 0 -10 2.41377 1.15167 #224#) 
COMMENT                             #226=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #163# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    3.90747 4.18258 1 0.082322) 0.8 1.8 0) 
COMMENT                             #227=(CLabel (CWidget 0 (0 0) 1 2 0 0 #185# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "blasticidin resistance" "@N" 1 14.9138 
COMMENT                                   -8.56966 0 -10 9.29221 1.15167 #226#) 
COMMENT                             #228=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #163# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.55837 5.05275 1 0.082322) 0.8 1.8 0) 
COMMENT                             #229=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 1413698627 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "EGFP" "@N" 1 
COMMENT                                   14.2359 1.95869 0 -10 2.88705 1.15167 #228#) 
COMMENT                             #230=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #163# 768 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    2.29332 2.88699 1 0.082322) 0.8 1.8 0) 
COMMENT                             #231=(CLabel (CWidget 0 (0 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ampicillin resistance" "@N" 1 -11.7913 
COMMENT                                   -12.3067 0 -10 8.97669 1.15167 #230#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #232=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -889 100) 
COMMENT                            (CObjectList 
COMMENT                             #233=(CScratch 
COMMENT                                   (CWidget 0 (3 #20# 0) 1 2 0 0 #171# 975782222 
COMMENT                                    100) (LOGPEN 0 10 16711680) 8 1 1.09012 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #234=(CLabel (CWidget 0 (0 0) 1 2 0 0 #186# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HIV-1 psi packaging signal" "@N" 1 -17.9297 
COMMENT                                   6.96932 0 -10 11.8795 1.15167 #233#) 
COMMENT                             #235=(CLine 
COMMENT                                   (CWidget 0 (3 #21# 0) 1 2 0 0 #171# 607 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 0.835356 0.546783 
COMMENT                                   0.708129) 
COMMENT                             #236=(CLabel (CWidget 0 (0 0) 1 2 0 0 #186# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HIV-1 Rev response element" "@N" 1 -14.135 
COMMENT                                   10.2962 0 -10 13.0312 1.15167 #235#) 
COMMENT                             #237=(CScratch 
COMMENT                                   (CWidget 0 (3 #23# 0) 1 2 0 0 #171# 101171880 
COMMENT                                    100) (LOGPEN 0 10 16711680) 8 1 3.85989 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #238=(CLabel (CWidget 0 (0 0) 1 2 0 0 #186# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "HIV-1 cPPT" 
COMMENT                                   "@N" 1 11.3004 -10.1385 0 -10 5.45858 1.15167 
COMMENT                                   #237#)) (CObjectList)) 
COMMENT                      #239=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -957 100) 
COMMENT                            (CObjectList 
COMMENT                             #240=(CScratch 
COMMENT                                   (CWidget 0 (3 #0# 0) 1 2 0 0 #184# 101623464 
COMMENT                                    100) (LOGPEN 0 6 10040064) 6 1 4.53217 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #241=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 982554374 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "V5 epitope" 
COMMENT                                   "@N" 1 15.4088 -2.59199 0 -10 4.9853 1.15167 
COMMENT                                   #240#)) (CObjectList)) 
COMMENT                      #242=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -1451 100) 
COMMENT                            (CObjectList 
COMMENT                             #243=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #182# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.835356 4.2998 4.49493 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #244=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 2081 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early promoter" "@N" 1 16.7421 -4.01204 
COMMENT                                   0 -10 9.29221 1.15167 #243#) 
COMMENT                             #245=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #182# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.992946 1.16597 1.39282 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #246=(CLabel (CWidget 0 (0 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "RSV/HIV-1 5' LTR" "@N" 1 -17.8191 4.54167 0 
COMMENT                                   -10 8.29831 1.15167 #245#) 
COMMENT                             #247=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #24# 0) 1 2 0 0 #182# 768 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    0.0055161 0.190305 1 0.082322) 0.8 1.8 0) 
COMMENT                             #248=(CLabel (CWidget 0 (0 0) 1 2 0 0 #185# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hCMVTRE promoter" "@N" 1 -5.67645 13.5623 0 
COMMENT                                   -10 9.19756 1.15167 #247#)) (CObjectList)) 
COMMENT                      #249=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -1240 100) 
COMMENT                            (CObjectList 
COMMENT                             #250=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #173# 0 100) 
COMMENT                                    (LOGPEN 0 6 128) 6 0.86448 4.14082 4.22809) 
COMMENT                                   0.4 0.082322 0.932791 1.8 1.8 0) 
COMMENT                             #251=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 982516374 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "synthetic EM7 promoter" "@N" 1 16.4659 
COMMENT                                   -6.92199 0 -10 10.6174 1.15167 #250#) 
COMMENT                             #252=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #187# 0 100) 
COMMENT                                    (LOGPEN 0 2 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    1.52244 1.58036 1 0.082322) 0.8 1.8 0) 
COMMENT                             #253=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 858993459 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 -17.4115 0.473288 0 -10 5.66368 
COMMENT                                   1.15167 #252#) 
COMMENT                             #254=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #173# 0 100) 
COMMENT                                    (LOGPEN 0 6 128) 6 0.866936 1.39174 1.479) 
COMMENT                                   0.4 0.082322 0.960308 1.8 1.8 0) 
COMMENT                             #255=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 982554610 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T3 promoter" 
COMMENT                                   "@N" 1 -16.8209 2.01053 0 -10 5.61635 1.15167 
COMMENT                                   #254#) 
COMMENT                             #256=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #19# 0) 1 2 0 0 #173# 0 100) 
COMMENT                                    (LOGPEN 0 6 128) 6 0.835356 3.41791 3.50518) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #257=(CLabel (CWidget 0 (0 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 7.20122 -17.9035 0 -10 5.61635 1.15167 
COMMENT                                   #256#)) (CObjectList)) 
COMMENT                      #258=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -819 100) 
COMMENT                            (CObjectList 
COMMENT                             #259=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #162# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    4.27774 4.33014 1 0.082322) 0.8 1.8 0) 
COMMENT                             #260=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 192 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 13.8458 -5.47941 0 -10 3.99139 1.15167 
COMMENT                                   #259#) 
COMMENT                             #261=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #162# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    2.97663 3.29104 1 0.082322) 0.8 1.8 0) 
COMMENT                             #262=(CLabel (CWidget 0 (0 0) 1 2 0 0 #185# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 origin" "@N" 
COMMENT                                   1 -1.94705 -14.3646 0 -10 3.67587 1.15167 
COMMENT                                   #261#) 
COMMENT                             #263=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #162# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    1.64311 1.92098 1 0.082322) 0.8 1.8 0) 
COMMENT                             #264=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 12632319 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -16.1516 -3.64242 0 -10 4.08605 1.15167 
COMMENT                                   #263#) 
COMMENT                             #265=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #18# 0) 1 2 0 0 #162# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    3.44067 3.49307 1 0.082322) 0.8 1.8 0) 
COMMENT                             #266=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 982554590 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 7.23132 -16.3821 0 -10 3.99139 1.15167 
COMMENT                                   #265#)) (CObjectList)) 
COMMENT                      #267=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -1240 100) 
COMMENT                            (CObjectList 
COMMENT                             #268=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #171# 101998816 
COMMENT                                    100) (LOGPEN 0 10 16711680) 8 1 3.63787 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #269=(CLabel (CWidget 0 (0 0) 1 2 0 0 #194# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 11.2975 -13.4654 0 
COMMENT                                   -10 7.77769 1.15167 #268#) 
COMMENT                             #270=(CScratch 
COMMENT                                   (CWidget 0 (3 #17# 0) 1 2 0 0 #171# 100919296 
COMMENT                                    100) (LOGPEN 0 10 16711680) 8 1 3.63787 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #271=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #194# 1062660810 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 10.4073 -14.8922 0 
COMMENT                                   -10 7.2413 1.15167 #270#) 
COMMENT                             #272=(CLine 
COMMENT                                   (CWidget 0 (3 #22# 0) 1 2 0 0 #171# 1040 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 0.835356 3.68682 
COMMENT                                   3.84886) 
COMMENT                             #273=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #194# 39423 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 73 27 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "RSV/HIV-1 dU3/3' LTR" "@N" 1 13.6085 
COMMENT                                   -11.9281 0 -10 10.3966 1.15167 #272#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #274=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1030 100) 
COMMENT                     (CObjectList 
COMMENT                      #275=(CLabel (CWidget 0 (15 #25# 0) 1 2 0 0 #150# 0 100) 
COMMENT                            (LOGPEN 0 0 8388608) 1 
COMMENT                            (LOGFONT 100 38 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 7941" 
COMMENT                            "LMBP 7941" 65535 0.173539 -3.04932 0 -10 7.36751 
COMMENT                            1.5303 Nil) 
COMMENT                      #276=(CLabel (CWidget 0 (15 #26# 0) 1 2 0 0 #150# 0 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 67 25 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 20-NOV-2015" 
COMMENT                            "Last modified: 20-NOV-2015" 65535 -0.173539 -5.107 
COMMENT                            0 -10 10.9803 0.993904 Nil)) (CObjectList)) 
COMMENT               #277=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -1522 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #278=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -608 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 85713176 
COMMENT       (CStringList) 85710136 85709856 (CObList)))
FEATURES             Location/Qualifiers
     misc_marker     2506..2547
                     /vntifkey="22"
                     /label=V5\epitope
     promoter        2967..3032
                     /vntifkey="30"
                     /label=synthetic\EM7\promoter
     CDS             268..927
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             1269..1574
                     /vntifkey="4"
                     /label=ccdB
     CDS             3033..3431
                     /vntifkey="4"
                     /label=blasticidin\resistance
     attenuator      1615..1739
                     /vntifkey="0"
                     /label=attR2
     attenuator      complement(35..159)
                     /vntifkey="0"
                     /label=attR1
     CDS             1771..2487
                     /vntifkey="4"
                     /label=EGFP
     promoter        2580..2862
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      2819..2894
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      3823..3897
                     /vntifkey="43"
                     /label=SV40\polyA\early
     promoter        7079..7407
                     /vntifkey="29"
                     /label=RSV/HIV-1\5'\LTR
     rep_origin      4326..4781
                     /vntifkey="33"
                     /label=f1\origin
     CDS             4912..5772
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      6313..6715
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        6807..6890
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        6954..6970
                     /vntifkey="30"
                     /label=T3\promoter
     terminator      complement(3823..3897)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      4033..4108
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(4142..4158)
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    7518..7562
                     /vntifkey="21"
                     /label=HIV-1\psi\packaging\signal
     misc_feature    8072..8305
                     /vntifkey="21"
                     /label=HIV-1\Rev\response\element
     terminator      3517..3751
                     /vntifkey="43"
                     /label=RSV/HIV-1\dU3/3'\LTR
     misc_feature    3501..3516
                     /vntifkey="21"
                     /label=HIV-1\cPPT
     promoter        8823..9090
                     /vntifkey="29"
                     /label=hCMVTRE\promoter
BASE COUNT     2431 a      2135 c      2295 g      2237 t 
ORIGIN
        1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg 
       61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac 
      121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca 
      181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg 
      241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt 
      301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt 
      361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat 
      421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg 
      481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt 
      541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac 
      601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg 
      661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg 
      721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc 
      781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt 
      841 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac 
      901 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa 
      961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc 
     1021 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg 
     1081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac 
     1141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg 
     1201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga 
     1261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg 
     1321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca 
     1381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg 
     1441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg 
     1501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt 
     1561 tctggggaat ataaatgtca ggctccgtta tacacagcca gtctgcaggt cgaccatagt 
     1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt 
     1681 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt 
     1741 gatatccagc acagtggcgg ccgctcgagt atggtgagca agggcgagga gctgttcacc 
     1801 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 
     1861 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 
     1921 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 
     1981 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 
     2041 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 
     2101 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 
     2161 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 
     2221 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 
     2281 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 
     2341 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 
     2401 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 
     2461 actctcggca tggacgagct gtacaaggag ggcccgcggt tcgaaggtaa gcctatccct 
     2521 aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagtttg gaattaattc 
     2581 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt 
     2641 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 
     2701 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 
     2761 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     2821 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 
     2881 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 
     2941 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca 
     3001 tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat 
     3061 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca 
     3121 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc 
     3181 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag 
     3241 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct 
     3301 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg 
     3361 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt 
     3421 gggagggcta agcacaattc gagctcggta cctttaagac caatgactta caaggcagct 
     3481 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 
     3541 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 
     3601 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 
     3661 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 
     3721 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 
     3781 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 
     3841 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     3901 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag 
     3961 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 
     4021 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 
     4081 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc 
     4141 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga 
     4201 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 
     4261 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 
     4321 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 
     4381 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 
     4441 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 
     4501 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 
     4561 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 
     4621 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 
     4681 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 
     4741 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga 
     4801 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 
     4861 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 
     4921 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 
     4981 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 
     5041 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 
     5101 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 
     5161 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 
     5221 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 
     5281 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 
     5341 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 
     5401 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 
     5461 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 
     5521 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 
     5581 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 
     5641 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 
     5701 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 
     5761 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 
     5821 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 
     5881 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 
     5941 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 
     6001 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 
     6061 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 
     6121 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 
     6181 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 
     6241 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 
     6301 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
     6361 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
     6421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
     6481 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
     6541 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 
     6601 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 
     6661 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 
     6721 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 
     6781 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 
     6841 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 
     6901 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac 
     6961 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct 
     7021 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc 
     7081 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac 
     7141 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat 
     7201 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg 
     7261 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 
     7321 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 
     7381 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg 
     7441 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg 
     7501 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag 
     7561 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat 
     7621 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag 
     7681 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca 
     7741 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata 
     7801 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga 
     7861 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc 
     7921 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata 
     7981 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag 
     8041 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag 
     8101 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat 
     8161 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc 
     8221 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa 
     8281 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca 
     8341 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc 
     8401 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct 
     8461 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata 
     8521 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat 
     8581 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag 
     8641 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga 
     8701 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat 
     8761 ccattcgatt agtgaacgga tctcgacggt atcgatggtc cgaggttcta gacgagttta 
     8821 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta 
     8881 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg 
     8941 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact 
     9001 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa 
     9061 gcagagctcg tttagtgaac cgtcagatcg caccggtc 
//
